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    Urea Agar Base

    Chinese Name:尿素酶琼脂基础
    Product No.:HB4095
    Specification:250g/500g
    Product Price:
    Product Use:Used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
    Remarks:

    Technical Data:

    Urea Agar Base is a medium used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.

    Approximate Formula:

    Ingredients

    gm/liter

    Peptic Digest of Animal Tissue

    1.0

    Sodium Chloride

    5.0

    Dextrose

    1.0

    Potassium Dihydrogen Phosphate

    2.0

    Phenol Red

    0.012

    Agar

    12.0

    Final pH6.8±0.2 at 25℃

    *Adjusted and/or supplemented as required to meet performance criteria.

    Directions:

    Suspend 21.0g of the medium in one liter of deionized or distilled water. Mix well and heat with frequent agitation. Boil for 1 minute. Dispense into tubes or flasks. Sterilize in the autoclave at 121℃(15 lbs.) for 15 minutes.

    Principle and Interpretation:

    When organisms utilize urea, ammonia is formed during incubation which makes the reaction of these media alkaline, producing a red-pink color. Consequently, urease production may be detected by the change in the phenol red indicator.

    Appearance:

    Dehydrated medium is a free-flowing light red powder. The prepared medium is a kind of orange-red transparent gel

    Precautions:

    This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.

    Storage conditions and Shelf life:

    Urea Agar must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

    Quality control:

    Prepare the culture medium as per label directions. Inoculate and incubate at 36±1℃ for 18-24 hours.

    Microorganism

    Strains Number

    Growth

    Remarks

    Escherichia coli

    ATCC 25922

    luxuriant

    Negative, turn yellow

    Salmonella typhimurium

    ATCC 14028

    luxuriant

    Negative, turn yellow

    Proteus vulgaris

    ATCC 13315

    luxuriant

    Postive, turn red

    Proteus mirabilis

    ATCC 12453

    luxuriant

    Postive, turn red

    Reference:

    1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

    2. Christensen. 1946. J. Bacteriol. 52:461.

    3. MacFaddin. 2000. Biochemical tests for identification of medical bacteria, 3rd ed. Lippincott Williams

    & Wilkins, Baltimore, Md.

    4. Rustigian and Stuart. 1941. Proc. Soc. Exp. Biol. Med. 47:108.


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    脑心浸液琼脂(BHI)
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    假单菌琼脂基础培养基/CN琼脂
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