Candida Chromogenic Medium-Product Details-qingdao hopebio
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    Candida Chromogenic Medium

    microbial medium
    Chinese Name:念珠菌显色培养基
    Product No.:HB7015
    Specification:1000ml
    Product Price:
    Product Use:Used for the isolation and identification of Candida, the colonies of Candida albicans are blue-green
    Remarks:

    Technical Data:

    Description: Used for the isolation and identification of Candida, the colonies of Candida albicans are blue-green. Candida chromogenic medium based on enzyme substrate technique, distinguish different Candida by colors, incubated at 25-28 ℃ for 48 hours, the colonies of Candida albicans are blue-green, Candida tropicalis are blue-gray or blue, Candida krusei are pink, other Candida are inhibited.

    Ingredients (g / L)

    Nutrients

    30.0

    Chromogenic reagent

    2.9

    Chloramphenicol

    0.1

    Agar

    15.0

    Final pH 6.0 ± 0.2 at 25

    *Adjusted and/or supplemented as required to meet performance criteria

    Directions:

    Suspend9.6g/200mL (adjust by proportion 4.8g/100mL), boil in one minute. Cooling the medium to 45-50 ℃ by in a water bath, , pour into sterile petri dishes (15ml for 9cm dish, 10mL for 7cm dish), after agar completely solidifying, put in incubator inverted at 36 ℃ ± 1 ℃ for 0.5-1 hours.

    Procedures:

    1. Prepare the sample solution according to national standards, SN standard, FDA standards or other methods;

    2. Take the sample solution and apply or streak it evenly on the plate;

    3. Incubate at 25-28 ℃ for 48h. the colonies of Candida albicans are blue-green, Candida tropicalis are blue or blue-gray, Candida glabrata are purple, Candida krusei are pink, other Candida are inhibited.

    4. Undetermined Candida albicans colonies can streak onto PDA agar plates, at 25-28 ℃ for 24-48 hours, pick single colony to do microscopy and biochemical tests.

    Quality Control:

    1. Appearance:

    The dehydrate powder medium is homogeneous, having good flow ability; milky white, prepared medium is colorless solid.

    2. Microbial sensitivity test:

    Incubated at 36 ± 1 18-24h:

    QC Strains

    Growth

    Colonies color

    Candida albicans

    +++

    Blue-green

    Candida tropicalis

    +++

    blue-gray, iron blue

    Candida krusei's

    +++

    Pink

    Candida glabrata

    +++

    purple

    Other Candida

    +++

    white

    Coliform

    -

    -

    Typical features:

    The typical colonies of Candida albicans are blue-green, Candida tropicalis are blue or blue-gray, Candida glabrata are purple, Candida krusei are pink, other Candida are inhibited.

    Storage:

    The prepared plates must be used immediately, avoid direct exposure to light. The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

    Failure:

    Dry powder media which are over the shelf life, caking and color variation cannot be used.

    Note:

    This medium is only for laboratory use.

    Reference:

    1. Mega et al (J. Biochem. vol. 71, pp 107-114, 1972). 
    2. Bendel et al. XP-002064132 "Distinct Mechanisms of Epithelial Adhesion for Candida albicans and Candida tropicalis" vol. 92, Oct. 1993, pp. 1840-1849. 
    3. Mira-Gutierrez et al., XP-002064133 "Identification of yeasts by hydrolysis of amides" Nov. 8, 1994, pp. 101-106. Crist, Jr. et al., XP-002064134 "Comparison of the MUREX C. Albicans, Albicans/Sure, and Bacticard Candida Test Kits with the Germ Tube Test for Presumptive identification of Candida albicans" vol. 36, Jul. 5, 1996, pp. 2616-2618. 


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