Baird Parker Agar Medium is used for the selective isolation and culture of Staphylococcus aureus.

Formula:

Pancreatic Digest of Casein....10.0
Beef Extract...............................5.0
Yeast Extract.............................1.0
Sodium Pyruvate.....................10.0
Glycine....................................12.0
Lithium Chloride........................5.0
Agar.........................................20.0
Final pH 6.8±0.2 at 25℃

Directions:

Suspend 58.0 grams of the product in 950ml of distilled water, dispensing into bottles of 95ml, sterilize in an autoclave at 121 ℃ for 15 minutes. Before use, heat to melt the agar and cool to about 50 ℃, each 95ml is add egg yolk tellurite potassium enrichment reagent (thawed at room temperature) 5 ml, shaking well. Storing in refrigerator shall not exceed 48 hours before use. 

Quality control and typical characteristics:

Culture at 36±1℃ for 45-48 hours, single colony of S. aureus is round, smooth, convex, moist, diameter 2-3mm. Dark gray to black, shiny, often has colored (non-white) edge, around it is opaque (precipitation) circle, a clear band outside.