用途:用于肠杆菌科细菌的选择性增菌培养。
成分(g/L)
Pancreatic Digest of Gelatin/明胶蛋白胨
|
10.0
|
Glucose monohydrate/葡萄糖
|
5.0
|
Disodium hydrogen phosphate dihydrate/磷酸氢二钠
|
8.0
|
Potassium dihydrogen phosphate/磷酸二氢钾
|
2.0
|
Dehydrated Ox Bile/牛胆盐
|
20.0
|
Brilliant Green/煌绿
|
0.015
|
pH值7.2±0.2
|
25℃
|
用法:
Suspend 45.0g/liter, and heat toboilingfor 1 to 2 minutes.Transfer 120-ml portionsto 250-ml volumetric flasks or9-ml portions to test tubes,all being capped with cotton plugs or loose-ftting caps.Heat at 100℃ for 30 min and cool immediately.
称取本品45.0g,加热搅拌溶解于1000ml蒸馏水中,分装三角瓶每瓶120ml或分装试管,每管9ml,100℃水浴加热30分钟,迅速在流动的冷水中冷却,无需高压灭菌。